Biosynthesis of the polygalacturonic acid chain of pectin by a particulate enzyme preparation from Phaseolus aureus seedlings.
نویسندگان
چکیده
منابع مشابه
Biosynthesis of the polygalacturonic acid chain of pectin by a particulate enzyme preparation from Phaseolus aureus seedlings.
Pectin is an important structural component of the cell walls of all higher plants and is formed primarily during the early stages of cellular growth.1 The basic building unit of this polymer is known to be D-galactopyranosyluronic acid in which the linear skeleton of these units is connected by a-1,4-D-glycosidic linkages.2 The carboxyl groups of this compound are methylated to various degrees...
متن کاملBiosynthesis of Alkali Insoluble Polysaccharide from UDP-d-Glucose with Particulate Enzyme Preparations from Phaseolus aureus.
A particulate enzyme system from Phaseolus aureus seedlings catalyzes the synthesis of alkali insoluble polysaccharide material from UDP-d-glucose. 80 to 90% of the d-glucose units are joined by beta-1,4 linkages, the remainder being combined by beta-1,3 linkages. It is not known whether the material is a single polysaccharide or a mixture.With a substrate concentration of 4 x 10(-3)m the enzym...
متن کاملMetabolism of D-glucuronic acid and D-galacturonic acid by Phaseolus aureus seedlings.
n-Glucuronic acid and its la&one are known to be converted to polysaccharides by plant tissues. Ripening strawberries have been shown to incorporate the intact carbon skeleton of D-ghcuronolactone into pectin (1). It has also been demonstrated that wheat plants and corn coleoptiles incorporate n-glucuronic acid and its lactone into the pentosyl moieties of pentosan with loss of carbon 6 (2,3). ...
متن کاملIn vitro stabilization and minimum active component of polygalacturonic acid synthase involved in pectin biosynthesis.
Polygalacturonic acid (PGA) synthase successively transfers galacturonic acid to oligogalacturonic acid by an alpha1,4-linkage to synthesize PGA, the backbone of plant pectic homogalacturonan. PGA synthase has not been purified to date due to its instability in vitro. In this study, we found stable conditions in vitro and separated a minimum active component of the enzymes from pea and azuki be...
متن کاملMetabolism of &lucuronic Acid and D-Galacturonic Acid by Phaseolus aureus Seedlings*
n-Glucuronic acid and its la&one are known to be converted to polysaccharides by plant tissues. Ripening strawberries have been shown to incorporate the intact carbon skeleton of D-ghcuronolactone into pectin (1). It has also been demonstrated that wheat plants and corn coleoptiles incorporate n-glucuronic acid and its lactone into the pentosyl moieties of pentosan with loss of carbon 6 (2,3). ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1965
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.54.6.1626